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A study conducted in 2013 found no evidence of a Khazar origin for Ashkenazi Jews and suggested that "Ashkenazi Jews share the greatest genetic ancestry with other Jewish populations, and among non-Jewish populations, with groups from Europe and the Middle East.No particular similarity of Ashkenazi Jews with populations from the Caucasus is evident, particularly with the populations that most closely represent the Khazar region. Pirooznia, Elhaik advanced the view that the first Ashkenazi populations to speak the Yiddish language came from areas near four villages in Eastern Turkey along the Silk Road whose names derived from the word "Ashkenaz", arguing that Iranian, Greek, Turkish, and Slav populations converted on that travel route before moving to Khazaria, where a small-scale conversion took place.In the case of Ashkenazi and Sephardi Jews (in particular Moroccan Jews), who are closely related, the source of non-Jewish admixture is mainly southern European.Behar and colleagues have remarked on an especially close relationship between Ashkenazi Jews and modern Italians, particularly Tuscans.In this view, analysis of Ashkenazi Jews together with a large sample from the region of the Khazar Khaganate would corroborate earlier results that Ashkenazi Jews derive their ancestry primarily from populations of the Middle East and Europe, that they possess considerable shared ancestry with other Jewish populations, and that there is no indication of a significant genetic contribution either from within or from north of the Caucasus region." In 2016, together with R. The study was dismissed by Sergio Della Pergola as a "falsification", claiming it failed to include Jewish groups such as the Italkim and Sephardic Jews, to whom Ashkenazi Jews are closely related genetically.

This is consistent with Jewish traditions in placing most Jewish paternal origins in the region of the Middle East.

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Studies of autosomal DNA, which look at the entire DNA mixture, have become increasingly important as the technology develops.

They show that Jewish populations have tended to form relatively closely related groups in independent communities, with most in a community sharing significant ancestry in common.

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